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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium

✍ Scribed by Rebekka Biedendieck; Martin Gamer; Lothar Jaensch; Steffen Meyer; Manfred Rohde; Wolf-Dieter Deckwer; Dieter Jahn


Book ID
118472359
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
564 KB
Volume
132
Category
Article
ISSN
0168-1656

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## Abstract A multiple vector system for the intracellular high‐level production of affinity tagged recombinant proteins in __Bacillus megaterium__ was developed. The N‐ and C‐terminal fusion of a protein of interest to a Strep II and a His~6~‐tag is possible. Corresponding genes are expressed unde