A submicroscopic homozygous deletion at the D3S3 locus in a cell line isolated from a small cell lung carcinoma

✍ Scribed by Dr. Pamela Rabbitts; Jonas Bergh; Jenny Douglas; Francis Collins; Jonathan Water

• Book ID: 102844496
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 691 KB
• Volume: 2
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870020312

✦ Synopsis

We have used 14 DNA probes, which detect 19 different restriction enzyme length polymorphisms, t o search for heterozygosity on chromosome 3 in five cell lines isolated from patients with small cell lung carcinoma. The cell lines on karyotype analysis did not show the deletion in chromosome 3 characteristic of this disease. Our objective was t o determine if allelic loss had occurred by some chromosomal mechanism other than deletion. Two of the cell lines are consistent with allelic loss having occured by whole chromosome loss and reduplication. The third may have lost only the short arm due t o i(3q) formation. The fourth cell line has an i(3q) chromosome, together with a translocation product involving the distal portion of the short arm of chromosome 3. Lack of evidence of heterozygosity for this distal portion of 3p suggests that a copy of the 3p homologue is involved in the translocation and therefore does not explain allelic loss of the other homologue. The fifth, while also likely t o have lost one chromosome homologue, has a submicroscopic deletion on all chromosome 3s, only detectable by RFLP analysis. Such homozygous deletions have recently proved useful in the isolation of tumour suppressor genes.

Cytogenetic Analysis

Cytogenetic analyses of the turnout cell lines were carried out after treatment of the cells with