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A subcutaneous mouse model for testing the virulence ofKlebsiellastrains

โœ Scribed by A. M. Simoons-Smit; A. M. J. J. Verweij-Van Vught; I. Y. R. Kanis; D. M. MacLaren


Publisher
Springer Netherlands
Year
1983
Tongue
English
Weight
137 KB
Volume
49
Category
Article
ISSN
0003-6072

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โœฆ Synopsis


In our study of the role of K antigens in phagocytosis we compared six E. coli K ยง strains (K1, K2, K23, K(A)28, K(A)?, K(?) with their K-mutants. The induction of the chemiluminescence of PMN's after stimulation with K + and K-strains was enhanced for all K-strains. The kinetics of chemiluminescence production was dependent on the method of opsonization. The differences in opsono-phagocytosis between K ยง and K-strains as measured by chemiluminescence could mostly be explained by the ability of the K -strains to use the classical complement pathway for opsonization. The K antigens did not influence the function of common pili as far as the mannose-sensitive haemagglutination of guinea-pig erythrocytes is concerned.


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