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A study of the effect of specific growth rate and acetate on recombinant protein production ofEscherichia coliJM107

✍ Scribed by Claire Turner; Malcolm E. Gregory; Michael K. Turner

Publisher: Springer Netherlands
Year: 1994
Tongue: English
Weight: 442 KB
Volume: 16
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00128620

No coin nor oath required. For personal study only.

✦ Synopsis

The growth of Escherichia coli in fed-batch and continuous culture is examined and results

show that a-amylase production is strongly dependent on specific growth rate (dilution rate) of the culture and production is greatest at an intermediate rate. Using continuous culture, it has also been found that the presence of acetate above a certain concentration reduces both ~tmax, and the production of recombinant protein.

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