𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A study of indirect methods of assay for diamine oxidase

✍ Scribed by M.James C. Crabbe

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
318 KB
Volume
75
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Methods

Direct spectrophotometric assay. This was carried out using p-dimethylaminomethylbenzylamine as substrate as previously described (1,111.

Indirect spectrophotometric assays. These were carried out as described (2-4,12). A Cary 118C UV-VIS spectrophotometer was used throughout this study.

Enzyme puriJication. Human placental diamine oxidase was prepared as previously described (I 1) to a final specific activity of 1.3 U .mg-I. Highly 676

πŸ“œ SIMILAR VOLUMES

A fluorometric method for assay of RNase
A fluorometric method for assay of RNase activity
✍ Richard C. Kamm; Albert G. Smith; Harold Lyons πŸ“‚ Article πŸ“… 1970 πŸ› Elsevier Science 🌐 English βš– 215 KB

Most of the methods currently available for the determination of RNase activity are dependent on the measurement of acid-soluble products released 'by hydrolysis of RNA. The major advantages of these methods are that they are sensitive, reproducible, and convenient for large numbers of samples (l-3)

Studies on epstein-barr virus-related an
Studies on epstein-barr virus-related antigens. I. Indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen
✍ T. Matsuo; T. Osato; S. Nishi; H. Hirai πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 French βš– 461 KB πŸ‘ 1 views

## Abstract A useful method for the detection and assay of Epstein‐Barr virus (EBV)‐related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble prote