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A Study of Immunoglobulin G Glycosylation in Monoclonal and Polyclonal Species by Electrospray and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

✍ Scribed by Julian A. Saba; Jeremy P. Kunkel; David C.H. Jan; Werner E. Ens; Kenneth G. Standing; Michael Butler; James C. Jamieson; Hélène Perreault

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
308 KB
Volume
305
Category
Article
ISSN
0003-2697

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✦ Synopsis

N-linked oligosaccharides were released from human and bovine polyclonal immunoglobulin G (IgG) obtained from commercial sources and also from a monoclonal IgG 1 secreted by murine B-lymphocyte hybridoma cells (CC9C10) grown under different serum-free conditions. These conditions differed according to their steady-state dissolved oxygen concentrations. This work is based on a previous quantitative study where released glycans were characterized by fluorophore-assisted carbohydrate electrophoresis (FACE) and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) (

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