✦ LIBER ✦

A study of bile canalicular contractions in isolated hepatocytes

✍ Scribed by M. James Phillips; Chikara Oshio; Mamoru Miyairi; Hart Katz; Charles R. Smith

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 739 KB
Volume: 2
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840020603

No coin nor oath required. For personal study only.

✦ Synopsis

Living hepatocytes observed under standard methods of cell isolation and culture show frequent active contractions of bile canaliculi when viewed by time-lapse cinephotomicrography. This report gives details of the methods used to show the contractions. In addition, the characteristics of the contraction pattern are defined and analyzed. The contractions are regular, canalicular contraction time (systole) lasts 60 sec, and the most probably interval between contractions is 5 1/2 min. The finding of a regular pattern of contraction suggests an ordered, programmed, and integrated mechanism which entails a high degree of communication and functional coordination between neighboring hepatocytes.

📜 SIMILAR VOLUMES

Carbon monoxide as a regulator of bile c

Carbon monoxide as a regulator of bile canalicular contractility in cultured rat hepatocytes

✍ Yuichi Shinoda; Makoto Suematsu; Yoshiyuki Wakabayashi; Tsuneharu Suzuki; Nobuhi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 413 KB

This study aimed to examine the mechanism(s) by which carbon monoxide (CO), a product of heme oxygenase reaction, controls the contractility of bile canaliculus (BC) in hepatocytes. When BCs associated with the couplet cells in cultured rat hepatocyte suspension were observed using time-lapse video

A study on glutathione in isolated hepat

A study on glutathione in isolated hepatocytes

✍ J. Högberg 📂 Article 📅 1975 🏛 Elsevier Science ⚖ 91 KB

Redistribution of canalicular cell membr

Redistribution of canalicular cell membrane enzyme in hepatocytes following their isolation and during early cell culture

✍ R Gratzl; R Lassacher; A Majcen; J Graf 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 221 KB

in this study, although it may not have been immediately tis C in which there were no changes in albumin, bilirubin, or prothrombin time during deterioration of APC amongst apparent from studying the group data in Figs. 2 and3. The statement by Dr. Fabbri et al. that the variability between patients

Praziquantel did not exhibit hepatotoxic

Praziquantel did not exhibit hepatotoxicity in a study with isolated hepatocytes

✍ F. Ebeid; H. Farghali; S. Botros; A. Metwally 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 274 KB

Characterization of the transport of a s

Characterization of the transport of a synthetic bile salt, iodinated cholyl-glycyl-tyrosine, in isolated cultured rat hepatocytes

✍ John C. Deutsch; Mieko M. Iwahashi; Eileen M. Sutherland; John Mapoles; Francis 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 723 KB

taken up primarily through a sodium-dependent transport system (1-3). Organic anions such as indocyanine green, along with unconjugated bile salts, appear to be taken up by a separate sodium-independent transporter (2, 4, 5). The two carriers have been tentatively identified as a 48-kD protein for s

Biosynthesis and intracellular transport

Biosynthesis and intracellular transport of a bile canalicular plasma membrane protein: Studies in vivo and in the perfused rat liver

✍ Michele Maurice; Michael J. Schell; Bernard Lardeux; Ann L. Hubbard 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 979 KB

B10 is an integral glycoprotein of the plasma membrane that is exclusively localized to the canalicular (apical) domain in normal rat hepatocytes but may be expressed on the basolateral (sinusoidal and lateral) membrane in pathophysiological situations. To understand how B10 may be localized to the