## Abstract In vivo rescue of a defective MSV genome from isolated cells of the rat neoplasm MSBโ1 was achieved by simple and direct methods: (1) by mixing of tissueโcultureโgrown MSBโ1 cells with tissue extracts or with leukemogenic virus preparations prior to intramuscular inoculations into neona
A study of angiogenesis factors from five different sources using a radioimmunoassay
โ Scribed by S. Shahabuddin; S. Kumar; D. West; F. Arnold
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 653 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0020-7136
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โฆ Synopsis
Abstract
The use of a radioimmunoassay (RIA) demonstrated that angiogenic factors from human and animal tumours, normal bovine retinas, myocardial infarcts, synovial fluid from patients with joint diseases and wound fluid shared common antigenic determinants. Values for angiogenesis factors expressed as ฮผg Walker tumour TAF/mg protein varied from 0.8 (wound fluid) to 207 (myocardial infarct). Activated macrophages produced an angiogenic factor which did not crossreact. Normal tissue extracts which were nonโangiogenic by the chicken chorioallantoic membrane assay also failed to crossโreact in the RIA. The antigenic similarity of angiogenic factors from such a wide variety of sources suggests that, in order to minimize therapeutic side effects, it would be best to use angiogenic agonists and antagonists locally.
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Immobilized metal-ion affinity gel electrophoresis (IMAGE) has been demonstrated to be an efficient tool to study the binding of proteins to chelated transition metals such as Cu(I1). IMAGE was exploited to isolate several lipases from different sources and so evaluate their affinities for iminodiac