Studies on the rescue of a defective msv genome using leukemogenic viruses from different sources
✍ Scribed by R. C. Ting; L. W. Law
- Publisher
- John Wiley and Sons
- Year
- 1970
- Tongue
- French
- Weight
- 792 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
In vivo rescue of a defective MSV genome from isolated cells of the rat neoplasm MSB‐1 was achieved by simple and direct methods: (1) by mixing of tissue‐culture‐grown MSB‐1 cells with tissue extracts or with leukemogenic virus preparations prior to intramuscular inoculations into neonatal mice; and (2) by direct inoculations of these cells into neonatal or older mice previously infected with leukemogenic viruses or infected with vertically transmitted virus. Serial in vivo transfers of the rescued pseudotype viruses MSV (MLV) and MSV(RLV) were accomplished with ease but difficulty was experienced with those pseudotype viruses rescued with leukemogenic viruses of the Gross type, MSV (GLV) from C58, C3Hf/Fg and SJL strain leukemic mice; MSV (RadLV) rescued through the use of extracts from X‐ray‐induced leukemias of C57BL/Ka mice behaved in a similar manner to the MSV(GLV) pseudotype viruses. When tumor induction occurred, the lesions were of similar morphology no matter what the pseudotype virus inoculated and were classified as “atypical”; granulomas.