✦ LIBER ✦

A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand

✍ Scribed by Su Fern Teng; Kenny Sproule; Abid Hussain; Christopher R. Lowe

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 317 KB
Volume: 12
Category: Article
ISSN: 0952-3499
DOI: 10.1002/(sici)1099-1352(199901/02)12:1<67::aid-jmr443>3.0.co;2-4

No coin nor oath required. For personal study only.

✦ Synopsis

An IgG-binding ligand library comprising 88 adsorbents based on a known lead compound (Li et al., 1998) was generated on an agarose solid phase. Individual members of the library were synthesized in two chemical steps using cyanuric chloride as the scaffold immobilized on the beaded support. The library was screened for binding of pure human IgG, whence selected ligands from the library were further assessed for specificity by the purification of IgG from human plasma. The potential of this strategy for the rapid identification and evaluation of chemical leads was demonstrated by the discovery of ligands with IgG binding capabilities. It was found that ligands comprising 3-aminophenol and an aminonaphthol moiety substiuted on a triazine nucleus generally performed better than other ligands in the library. An immobilized ligand 22/8 adsorbent was able to purify IgG with high yield and a purity b99% from diluted human plasma.

📜 SIMILAR VOLUMES

Design, Synthesis, and Biological Evalua

Design, Synthesis, and Biological Evaluation of a Scaffold for iGluR Ligands Based on the Structure of (-)-Dysiherbaine.

✍ Jamie L. Cohen; Agenor Limon; Ricardo Miledi; A. Richard Chamberlin 📂 Article 📅 2006 🏛 John Wiley and Sons ⚖ 30 KB 👁 2 views

Combinatorial de novo design and applica

Combinatorial de novo design and application of a biomimetic affinity ligand for the purification of human anti-HIV mAb 4E10 from transgenic tobacco

✍ Dimitris Platis; Anastasios Maltezos; Julian K-C. Ma; Nikolaos E. Labrou 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 509 KB

## Abstract Monoclonal anti‐HIV antibody 4E10 (mAb 4E10) is one of the most broadly neutralizing antibodies against HIV, directed against a specific epitope on envelope protein gp41. In the present study, a combinatorial __de novo__ design approach was used for the development of a biomimetic ligan

Synthesis of the Four Stereoisomers of 1

Synthesis of the Four Stereoisomers of 1-Amino-2-(hydroxymethyl)cyclobutanecarboxylic Acid and Their Biological Evaluation as Ligands for the Glycine Binding Site of the NMDA Receptor

✍ Claus-Jürgen Koch; G. Höfner; Kurt Polborn; Klaus Theodor Wanner 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 194 KB 👁 1 views

ChemInform Abstract: The Azomethine Ylid

ChemInform Abstract: The Azomethine Ylide Strategy for β-Lactam Synthesis. An Evaluation of Alternative Pathways for Azomethine Ylide Generation.

✍ Giles A. Brown; Sarah R. Martel; Richard Wisedale; Jonathan P. H. Charmant; Neil 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 32 KB 👁 1 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

ChemInform Abstract: Synthesis and Bindi

ChemInform Abstract: Synthesis and Binding Affinity of 2-Phenylimidazo[1,2-a]pyridine Derivatives for Both Central and Peripheral Benzodiazepine Receptors. A New Series of High-Affinity and Selective Ligands for the Peripheral Type.

✍ G. TRAPANI; M. FRANCO; L. RICCIARDI; A. LATROFA; G. GENCHI; E. SANNA; F. TUVERI; 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 33 KB 👁 1 views