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A straightforward method for the simultaneous preparation of radiolabeled l-dihydroörotic and N-carbamyl-l-aspartic acids

✍ Scribed by Thomas W. Kensler; Nyun Han; David A. Cooney

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
380 KB
Volume
111
Category
Article
ISSN
0003-2697

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✦ Synopsis

A straightforward method is presented for the simultaneous enzymatic preparation of radiolabeled L-dihydroiirotic and N-carbamyl-L-aspartic acids. [carboxytT]Orotic acid is incubated in the presence of excess NADH to drive the reaction catalyzed by L-dihydroiirotate dehydrogenase in the reverse direction, forming L-[Y]dihydroLirotic acid. The contamination of the commercial reagent enzyme, L-dihydrohrotate dehydrogenase from Zymobacterium oroticum,

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