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A split-ubiquitin-based assay detects the influence of mutations on the conformational stability of the p53 DNA binding domain in vivo

โœ Scribed by Nils Johnsson


Book ID
117104805
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
195 KB
Volume
531
Category
Article
ISSN
0014-5793

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## Abstract Tumor suppressor protein p53 becomes inactive due to mutation on its DNA binding core domain leading to misbehavior of this protein and preventing its interaction with DNA. In the present study, changes of the protein conformation by five hot spot mutations of T-p53C were assessed preve