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A spectroscopic-based laboratory experiment for protein conformational studies

✍ Scribed by Carlos Henrique I. Ramos

Book ID
101752803
Publisher
The American Society for Biochemistry and Molecular Biology
Year
2004
Tongue
English
Weight
144 KB
Volume
32
Category
Article
ISSN
1470-8175

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✦ Synopsis

Abstract

This article describes a practical experiment for teaching basic spectroscopic techniques to introduce the topic of protein conformational change to students in the field of molecular biology, biochemistry, or structural biology. The spectroscopic methods employed in the experiment are absorbance, for protein concentration measurements, and fluorescence and circular dichroism, for probing protein conformational changes.

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