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A Spectrophotometric Method for Assay of Tannase Using Rhodanine

โœ Scribed by Shweta Sharma; T.K. Bhat; R.K. Dawra

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
59 KB
Volume
279
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible.

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