A Spectrophotometric Assay for the Detection of 2-Aminophenols in Biological Samples

✍ Scribed by N.H.P. Cnubben; B. Blaauboer; S. Juyn; J. Vervoort; I.M.C.M. Rietjens

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 522 KB
• Volume: 220
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1314

✦ Synopsis

A sensitive and efficient spectrophotometric assay is described for the determination of 2 -aminophenols in biological samples. Using ferric ions as a metal catalyst, 2 -aminophenol dimerizes in an acidic aqueous environment to 2-hydroxyisophenoxazin-3-one, an intensively colored dye. The newly developed assay is suitable for the detection of 2 -aminophenols in the micromolar range. The paper demonstrates that this chemical assay is also applicable for the determination of 2 -aminophenols substituted with a halogen at the ortho, meta, or para position of the aromatic ring with respect to the amino moiety, (\lambda_{\max }), and the molar extinction coefficient varying with the substituent pattern. Results obtained for fluorinated 2 -aminophenol detection in biological samples by either this new method or ({ }^{19} \mathrm{~F}) NMR are similar. This observation corroborates that the newly developed assay is suitable for detection of 2 -aminophenols in biological samples. c 1994 Academic Press, Inc.