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A Specific Complex between a Restriction Endonuclease and its DNA Substrate

โœ Scribed by Robert Yuan and Matthew Meselson


Book ID
123649297
Publisher
National Academy of Sciences
Year
1970
Tongue
English
Weight
249 KB
Volume
65
Category
Article
ISSN
0027-8424

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A technique for radiolabeling DNA restri
โœ Andrew P. Feinberg; Bert Vogelstein ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 973 KB

A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligon