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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity


Book ID
107712044
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
105 KB
Volume
137
Category
Article
ISSN
0003-2697

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A technique for radiolabeling DNA restri
✍ Andrew P. Feinberg; Bert Vogelstein πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 973 KB

A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligon