A technique for radiolabeling DNA restri
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Andrew P. Feinberg; Bert Vogelstein
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Article
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1983
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Elsevier Science
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English
β 973 KB
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligon