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A somatic cell approach to the genetic analysis of the laboratory mouse

✍ Scribed by Kozak, Christine ;Nichols, Elizabeth A. ;Ruddle, Frank H.

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 241 KB
Volume: 187
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401870214

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The determination of gene linkage in the laboratory mouse was approached through somatic cell techniques. Cells of an established line of Chinese hamster lung fibroblasts, E‐36, were fused with a population of mouse macrophages obtained from the AHe/J inbred strain. A total of 62 primary and secondary clones were isolated in HAT selective medium and characterized for the segregation of 12 murine enzyme markers by starch gel electrophoresis. While all other enzymes segregated nonrandomly, GPI was generally retained. Twelve subclones were isolated in medium containing 8‐azaguanine to determine whether this retention was due to a syntenic relationship between GPI and the enzymes of the salvage pathway of purine biosynthesis necessary for growth in HAT, but were found to express murine GPI. Of the other enzymes examined, MOD and MPI were determined to be syntenic and the others segregated independently of each other.

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