Solid-phase ELISAs for the determination of EGF receptor (EGF-R) and pp60c-src tyrosine protein kinase activity are described. The methods were developed and optimized using purified recombinant EGF-R intracellular domain (ICD) and pp60c-src tyrosine protein kinases. A standardized assay that utiliz
โฆ LIBER โฆ
A Solid-Phase Assay for the Determination of Protein Tyrosine Kinase Activity of c-src Using Scintillating Microtitration Plates
โ Scribed by Albert F. Braunwalder; Donna R. Yarwood; Tony Hall; Martin Missbach; Kenneth E. Lipson; Matthew A. Sills
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 88 KB
- Volume
- 234
- Category
- Article
- ISSN
- 0003-2697
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Protein tyrosine phosphatases are a class of enzymes that function to modulate tyrosine phosphorylation of cellular proteins and play an essential role in regulating cell function. PTP1B has been implicated in the negative regulation of the insulin signaling pathway by dephosphorylating the activate