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A solid-phase assay for the activity of CMPNeuAc:Gal β1-4GlcNAc-R α-2,6-sialyltransferase

✍ Scribed by Sharon Mattox; Kathryn Walrath; Debbie Ceiler; David F. Smith; Richard D. Cummings


Book ID
107719071
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
759 KB
Volume
206
Category
Article
ISSN
0003-2697

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A fluorescent assay for UDP-GlcNAc: Galp1,3GalNAc-R \(\boldsymbol{\beta 1 , 6 - \boldsymbol { N } \text { -acetylglucosaminyltransferase (core } 2}\) GlcNAc-T) activity has been developed involving dansylation of the enzyme reaction product. Core 2 GlcNAc-T detection was performed using unlabeled UD