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A Method for Determination of UDP-GlcNAc:GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man]β1-4N-acetylglucosaminyltransferase VI Activity Using a Pyridylaminated Tetraantennary Oligosaccharide as an Acceptor Substrate

✍ Scribed by Tomohiko Taguchi; Tomoya Ogawa; Ken Kitajima; Sadako Inoue; Yasuo Inoue; Yoshito Ihara; Yoshihiro Sakamoto; Kaoru Nagai; Naoyuki Taniguchi


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
68 KB
Volume
255
Category
Article
ISSN
0003-2697

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A fluorescent assay for UDP-GlcNAc: Galp1,3GalNAc-R \(\boldsymbol{\beta 1 , 6 - \boldsymbol { N } \text { -acetylglucosaminyltransferase (core } 2}\) GlcNAc-T) activity has been developed involving dansylation of the enzyme reaction product. Core 2 GlcNAc-T detection was performed using unlabeled UD