Extra-cellular production of a novel galactose oxidase from Fusarium acuminatum using submerged fermentation was studied. Glucose (1.0% w/v) was used as the sole carbon source. Maximum galactose oxidase production (approximately 4.0 U/ml) was obtained when fermentation was carried out at 25 ยฐC, with
A simplified purification procedure for galactose oxidase
โ Scribed by Paul Tressel; Daniel J. Kosman
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 664 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
Methods
for growing the fungus, Dactylium dendroides, and for purifying the secreted protein, galactose oxidase, are described. Fungal growth takes 1 week. Enzyme purification takes 2 days and yields 70 mg homogeneous enzyme per 25 liters growth medium.
๐ SIMILAR VOLUMES
A simple, quick procedure for the isolation of pig kidney o-amino acid oxidase [EC 1.4.3.3; o-amino acid:oxygen oxidoreductase (deaminating)] is described based upon the use of granulated hydroxylapatite for chromatography. The purity appears to be comparable to that obtained by other procedures. Th
## Abstract Sequential extraction of plant materials with methanolโchloroformโwater and phenolโacetic acidโwater mixtures gave good yields of waterโsoluble lowโmolecularโmass substances, lipids, proteins and polysaccharides in separate fractions unlaboriously and with little chemical damage. Result