## Abstract Mitochondrial respiration is inhibited in cells exposed to hypoxia, and the oxidation of NADH to NAD^+^ is blocked. As a result, oxidation reactions requiring NAD^+^ are blocked, disrupting cellular metabolism. We studied the influence of methylene blue, which oxidizes NADH, on hypoxic
โฆ LIBER โฆ
A Simplified Model of Hypoxic Injury in Primary Cultured Rat Hepatocytes
โ Scribed by Tomoo Kamiya, A-Hon Kwon, Toshiki Kanemaki, Yoichi Matsui, Shouji Uetsuji, Tadayoshi Okumura and Yasuo Kamiyama
- Book ID
- 125624632
- Publisher
- Society for In Vitro Biology
- Year
- 1998
- Tongue
- English
- Weight
- 532 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1475-2689
- DOI
- 10.2307/4294734
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Following prolonged hypoxia, mammalian cells invoke adaptive mechanisms to enhance oxygen delivery and promote energy conservation. We previously reported that hepatocytes subjected to prolonged moderate hypoxia (PO2 = 20-50 mmHg for > 3 hours) demonstrated a reversible inhibition of cellular respir
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