A Simplified Method for the Determination of Total Oxalate-Soluble Pectin Content in Japanese Pear
โ Scribed by Panmanas Sirisomboon; Munehiro Tanaka; Shuji Fujita; Takayoshi Akinaga; Takayuki Kojima
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 139 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0889-1575
No coin nor oath required. For personal study only.
โฆ Synopsis
The oxalate-soluble pectin is related to fruit "rmness with respect to the chelation of calcium ions with carboxyl groups of adjacent polyuronide chains. However, the accurate total oxalatesoluble pectin content is not easily attainable. Using the Japanese pear fruits (Pyrus serotina Rehder var. culta &Housui') as our model, we proposed a simple method to de"ne extraction time of total oxalate-soluble pectin in fruit without repetition based on half-extraction time which is the time when oxalate-soluble pectin was extracted up to 50%. There were two stages of extraction: primary-and secondary-extraction stages. The di!erent picking dates did not a!ect the extraction rates and the unaccomplished ratios in the primary stage in which half-extraction time embedded but did a!ect in the secondary stage (P(0.01). The extraction time of total oxalate-soluble pectin could also be estimated. The method recommended that the extraction should be done until half-extraction time was reached and the total oxalate-soluble pectin could be estimated by multiplying the pectin content at half-extraction time by two. We also postulate that the method may prove useful for other fruit and vegetables as well. Half-extraction time of Japanese pear was 2.1 h.
๐ SIMILAR VOLUMES
## Abstract Hyperhomocysteinemia is a risk factor for different diseases. Several methods have been developed to analyze homocysteine and the immunometric ones, although expensive, they are in widespread use. A rapid LCโMS/MS method for homocysteine assay has been developed for the application of l