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A simplified method for cyanogen bromide activation of agarose for affinity chromatography

✍ Scribed by Steven C. March; Indu Parikh; Pedro Cuatrecasas

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
237 KB
Volume
60
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

Affinity purification methods. Improved
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The high solubility of cyanogen bromide in N-methyl-2-pyrrolidone has afforded an efficient means of controlling the amount of the reagent used to activate an agarose preparation. By controlling the activation step, one can determine the final concentration of the leash moiety. The upper limits of ~

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## Abstract A new methodology for the rapid assessment of affinity ligands synthesized by combinatorial solid‐phase chemistry is reported. This screening strategy utilizes the target protein conjugated to FITC, and represents an almost universal technique for the preliminary screening of solid‐phas