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A simplified method for analysis of inorganic phosphate in the presence of interfering substances

โœ Scribed by Gary L. Peterson

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
546 KB
Volume
84
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new phosphate analysis method is described which is designed primarily for use with routine phosphatase assays. The method is modified extensively from that of Fiske and SubbaRow [(1925) J. Bid. C&em, 66, 3753 by decreasing 10fold the level of reducing agent, ANSA, increasing the concen~ation of ammonium molybdate to 4 mM, utilizing HCI as the only acid present, and incorporating SDS. The method is insensitive to pH, ATP, proteins, and detergents at concentrations normally encountered in enzyme purification and analysis experiments. The color developed is fivefold more stable than that from the originat Fiske-SubbaRow method, and the standard curve retains linearity for at least 24 hr with acid concentrations between 0.6 and 1.0 N HCI and for waveIen@hs between 500 and 900 nm. The simplicity, stability, and general applicability of the method allow for convenient and rapid analysis of large numbers of samples under a wide variety of conditions.

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