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A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells

โœ Scribed by D.C. West; A. Sattar; S. Kumar

Book ID
107712181
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
705 KB
Volume
147
Category
Article
ISSN
0003-2697

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A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers
โœ Thomas Sorger; Ralph J. Germinario ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 227 KB

A procedure for the direct solubilization of fibroblast monolayers allowing precise determination of DNA and protein content has been developed. This simple procedure is applicable to the analysis of these macromolecules in fibroblast cultures containing small numbers of cells (<l x 105).