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A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers

✍ Scribed by Thomas Sorger; Ralph J. Germinario

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 227 KB
Volume: 131
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90163-x

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✦ Synopsis

A procedure for the direct solubilization of fibroblast monolayers allowing precise determination of DNA and protein content has been developed. This simple procedure is applicable to the analysis of these macromolecules in fibroblast cultures containing small numbers of cells (<l x 105).

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