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A simple technique for purification of fibrinogen from plasma by affinity chromatography on ristocetin-agarose

✍ Scribed by Koji Suzuki; Junji Nishioka; Senichiro Hashimoto


Book ID
119034297
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
663 KB
Volume
18
Category
Article
ISSN
0049-3848

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Purification of a UTP: d-Glucose-1-phosp
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UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characte