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A simple spectrophotometric assay for arogenate dehydratase

✍ Scribed by Suhail Ahmad; Roy A. Jensen

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
404 KB
Volume
163
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple spectrophotometric assay for arogenate dehydratase, tile enzyme that catalyzes the formation of L-phenylalanine from L-arogenate, is presented. The method couples the arogenate dehydratase reaction with that of an aromatic aminotransferase partially purified from Acinetobacter calcoaceticus. In the presence of 2-ketoglutarate, phenylpyruvate formation is measured at 320 nm at basic pH. The method was compared with two other methods already in use in our laboratory for arogenate dehydratase. The new method is simple, quick, fairly sensitive, and especially suitable for the screening of a large number of samples. o 1987 Academic FTes, Inc.

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