A simple radioactivity recording system for column chromatography of 32P-labeled products

✍ Scribed by B.R. Jordan

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 216 KB
• Volume: 35
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(70)90031-x

✦ Synopsis

It is desirable in many applications to monitor and record continuously the radioactivity of the effluent from a chromatographic column rather than to count aliquots of the collected fractions. Several instruments designed for this purpose have been described (l-6)) and commercial apparatus is available. This is generally designed for tritium and carbon-14 counting, based on the liquid scintillation technique, and is consequently rather complex and expensive. If it is desired only to detect high-energy beta emitters, a much simpler system can be built around a GM counter. Considering the widespread use of szP, in particular in the nucleic acid field, such a device can be very useful. In this paper I describe a simple radioactivity recording system which has been in use in our laboratory for over a year and has significantly reduced the labor involved in routine preparation of 52P-labeled fractions.