𝔖 Bobbio Scriptorium
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A simple procedure to obtain continuous cell lines from bovine peripheral blood leucocytes

✍ Scribed by M.O. Asagba; Y.K. Ssentongo; R.H. Johnson; J.R. Smith


Book ID
119098725
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
805 KB
Volume
2
Category
Article
ISSN
0165-2427

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A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional sl