A simple procedure for isolation of Bufo arenarum C3 complement fraction and preparation of antiserum
β Scribed by Ricardo J. Llanos; Dora C. Miceli; Jorge N. Valz-Gianinet; Cecilia M. Whitacre
- Book ID
- 117567666
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 131 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0742-8413
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A simple nonenzymatic procedure is described for the separation of DNA, RNA, and proteins of cultured animal cells. DNA isolated by this procedure is large molecular weight and double stranded.
## Abstract (lβ^14^C)βdodecanal was synthesized from (lβ^14^C)βdodecanoic acid. The twoβstep synthesis beginning with 0.9 mg of the acid resulted in an overall yield of 42 % after purification. The final product was separated from the starting material and side products by silicic acid column chrom