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A simple polyacrylamide gradient gel preparation for estimating molecular weights

✍ Scribed by K. Lorentz

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 690 KB
Volume: 76
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90279-7

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✦ Synopsis

A simple device for the preparation of continuous linear gradients in usual gel rods is described. It consists of two-tube-supporting disks of plastic on an axis, forming a rotating rack. Its versatile applicability allows the preparation of concentration, pH, buffer, and buffer strength gradients as well. The exact percentage of transmission at any point on the gel (or the param eter wanted) can be predicted before the run. due to the densitometric determinations of a stable marker. Moreover linearity, slope, and length are controlled by the same measurement. The use of the procedure reported is demonstrated by estimating molecular weights of proteins between 20.000 and 300.000 daltons.

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