𝔖 Bobbio Scriptorium
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A simple PCR method for rapidly detecting defined point mutations

✍ Scribed by Jean-François Humbert; Loïc Elard


Book ID
114392992
Publisher
Elsevier
Year
1997
Weight
103 KB
Volume
2
Category
Article
ISSN
1366-2120

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Communicated by D a d L. Rimoin We present a simple screening method for detecting a known point mutation, using only one 5 'biotinylated oligonucleotide primer, with its 3' end adjacent to the mutation site. In parallel reactions, an amplified DNA template encompassing the biotinylated oligonucleot