We have developed a rapid and highly sensitive method for the detection of mutant K-ras codon 12 allele in the presence of 10 5 copies of the wild-type alleles. This sensitivity is achieved by selective amplification of mutant K-ras sequences, using a two-stage procedure with modified primers. In th
COLD PCR HRM: a highly sensitive detection method for IDH1 mutations
✍ Scribed by Blandine Boisselier; Yannick Marie; Marianne Labussière; Pietro Ciccarino; Virginie Desestret; XiaoWei Wang; Laurent Capelle; Jean-Yves Delattre; Marc Sanson
- Book ID
- 102259679
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 346 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1059-7794
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## Detection of molecular features such as K-ras mutations has been used to evaluate potential tumour markers in a wide variety of clinical samples. Here we have applied a recently developed highly sensitive method for detection of K-ras codon 12 mutations to colorectal and pancreatic cancer diagno