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A simple, one-step purification of cytochrome b from the bc1 complexes of bacteria

✍ Scribed by William E. Payne; Bernard L. Trumpower


Book ID
115920048
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
927 KB
Volume
213
Category
Article
ISSN
0014-5793

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NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimm