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A simple method of quantitative recovery of nucleotides from thin layers

โœ Scribed by Michael F. Turchinsky; Lidia P. Shershneva

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
202 KB
Volume
54
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple method for quantitative recovery of w-absorbing compounds from thin layers is described. The method consists in fixation of the appropriate area of adsorbent with nitrocellulose solution. After drying the fixed spots get detached from the glass as solid platelets. Therefore it becomes easy to perform subsequent quantitative procedures like elution, radioactivity counting, etc.

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Quantitative extraction, separation, and
Quantitative extraction, separation, and recovery of adenine-derived radioactivity in bases, nucleosides, and nucleotides from blood platelets using PEI-cellulose thin-layer chromatography
โœ Ulrich E. Honegger; Stephan S. Bogdanov; Peter R. Bally ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 887 KB

A method is reported for the separation and recovery of radioactivity in purine compounds, in particular in 3'5.CAMP derived from [llC]adenine-prelabeled blood platelets. It is based on Randerath's thin-layer ion-exchange chromatography on PEI-cellulose and allows simple, reproducible, and quantitat