Quantitative extraction, separation, and recovery of adenine-derived radioactivity in bases, nucleosides, and nucleotides from blood platelets using PEI-cellulose thin-layer chromatography
✍ Scribed by Ulrich E. Honegger; Stephan S. Bogdanov; Peter R. Bally
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 887 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is reported for the separation and recovery of radioactivity in purine compounds, in particular in 3'5.CAMP derived from [llC]adenine-prelabeled blood platelets. It is based on Randerath's thin-layer ion-exchange chromatography on PEI-cellulose and allows simple, reproducible, and quantitative estimation of radioactivity in most adenine-related purine derivatives. Up to 50 ~1 of neutralized perchloric acid cell extract (supematant) can be applied without further treatment to the thin layers. The recovery of added radioactivity as '"C-labeled bases. nucleosides, and nucleotides is 98-100%. Recovery of tritium-labeled compounds appears to vary with their charge and position of label, and is generally not as reproducible. One-and two-dimensional techniques are described.