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A simple method for typing clinical isolates of herpesvirus simplex: replication in the presence of 2h-1,3-oxazine-2,6 (3h)-dione (oxauracil)

✍ Scribed by Lawton A. Seal; Richard M. Jamison

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
449 KB
Volume
6
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A rapid simple method for the typing of wild HSV isolates based on selective inhibition of HSV‐2 replication by the pyrimidine analogue oxauracil (2H‐1,3‐oxazine‐2,6(3H)‐dione) has been developed. Chi‐square analysis of the results of typing of 30 wild strains by serum neutralization tests, immunofluorescence, and oxauracil‐inhibition indicates no significant difference in assignment of types to the isolates. The oxauracil‐inhibition test obviates confusion of typing resulting from use of immune sera containing cross‐reactive antibodies. We conclude oxauracil typing is a simple, rapid, and precise test which can be performed in any laboratory capable of isolating HSV.

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Evaluation of the effectiveness of 2H-1, 3-Oxazine-2,6(3H)-dione (Oxauracil) in the treatment of herpes simplex virus (HSV)-Induced acute encephalitis in mice
✍ Lawton A. Seal; Richard M. Jamison 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 292 KB

Treatment of suckling mice with the pyrimidine analogue 2H-1,3-oxazine-2,6(3H)-dione (oxauracil) proved successful in reducing mortality associated with HSV-2-induced encephalitis. Oxauracil did not decrease mortality associated with HSV-1 infection; however, there was an increased survival time and