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A Simple Method for the Reconstitution of Membrane Proteins into Giant Unilamellar Vesicles

✍ Scribed by Armelle Varnier; Frédérique Kermarrec; Iulia Blesneac; Christophe Moreau; Lavinia Liguori; Jean Luc Lenormand; Nathalie Picollet-D’hahan

Book ID
105926782
Publisher
Springer
Year
2010
Tongue
English
Weight
377 KB
Volume
233
Category
Article
ISSN
0022-2631

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Variations on the “dilution” method for
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✍ Jorge Ramírez; Martha Calahorra; Antonio Peña 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 575 KB

A method for the rapid incorporation of cytochrome c oxidase into membranes has been developed. This method essentially consists of obtaining a preparation of the enzyme in which it is isolated and then dissolving it in a medium containing 0.5% of the detergent Tween 20, which gives a final concentr