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A simple method for the isolation of neutral glycopeptides by affinity chromatography

โœ Scribed by Tom Krusius

Book ID
115906672
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
318 KB
Volume
66
Category
Article
ISSN
0014-5793

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โœ Dr. J. Simon; S. Benyhe; J. Hepp; E. Varga; K. Medzihradszky; A. Borsodi; M. Wol ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 563 KB

A kappa-opioid receptor subtype was purified from a digitonin extract of frog brain membranes, using affinity chromatography. The affinity resin was prepared by coupling dynorphin (1-10) to AH Sepharose 4B. The purified receptor binds 4,750 pmol [3H]ethylketocyclazocine (EKC) per mg protein (5,600-f