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A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations

✍ Scribed by A. Villanueva; J. C. Stockert; R. Armas-Portela

Publisher
Springer
Year
1984
Tongue
English
Weight
214 KB
Volume
81
Category
Article
ISSN
1432-119X

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✦ Synopsis

This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of "in situ" complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described.

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