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Reactive pyrolysis-GC: A simple, one-step, quantitative method for the analysis of fatty acids in natural products

✍ Scribed by Robert R. Freeman; Tetsuro Yuzawa; Chu Watanabe


Publisher
Wiley (John Wiley & Sons)
Year
2011
Tongue
English
Weight
559 KB
Volume
23
Category
Article
ISSN
0956-666X

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✦ Synopsis


Abstract

Fatty acids in a diverse array of sample types can be derivatized and profiled using reactive pyrolysis – gas chromatography (RxPY), which is, in essence, thermally assisted hydrolysis and methylation. Both solid and liquid samples are analyzed directly. When m‐trifluorophenyltrimethyl ammonium hydroxide is used as the organic alkali, analytical precision is on the order of 1%RSD. Quantitative data using ether standard addition or external standard yield accuracies within 5%. The analysis of omega‐3 acids in krill oil is used to illustrate the benefits of using RxPY‐GC.