A Simple Method for Quantification of Peptides and Proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
β Scribed by Park, Kyung Man; Bae, Yong Jin; Ahn, Sung Hee; Kim, Myung Soo
- Book ID
- 118005007
- Publisher
- American Chemical Society
- Year
- 2012
- Tongue
- English
- Weight
- 274 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0003-2700
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) was used to analyze the protein composition in several common and durum wheat varieties. Mass spectra were obtained directly from crude and partially puriΓed wheat gliadin and reduced glutenin subunit fractions. Mass spectra of
A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, mo
Different buffers typically used in biological applications were evaluated for compatibility with matrix-assisted laser desorptiodionization m a s spectrometry (MALDI-MS) measurements. It was found that the buffers recommended by Good and co-workers, such as HEPES and MOPS in concentrations below 5