A rapid and continuous spectrophotometri
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Tomás López Arnaldos; Matías López Serrano; Antonio A. Calderón; Romualdo Muñoz
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Article
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1999
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John Wiley and Sons
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English
⚖ 75 KB
An improved p-nitrophenyl-b-O-D-glucopyranoside method to measure b-glucosidase (EC. 3.2.1.21) activity is presented in which a cyclodextrin is added to the enzymatic mixtures. The inclusion complex consisting of p-nitrophenol and a-cyclodextrin has a higher e than p-nitrophenol alone, thus increasi