𝔖 Bobbio Scriptorium
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A simple immunofluorescent technique for the detection of human rotavirus

✍ Scribed by R. B. Moosai; P. S. Gardner; J. D. Almeida; M. A. Greenaway

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
380 KB
Volume
3
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed.

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