A simple gradient maker for use with zonal rotors
β Scribed by R.H. Hinton; M. Dobrota
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 653 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple adapter for reducing the chamber volume of B-XIV-type rotors is described. The adapter consists of a disk-like body, which occupies the lower part of the rotor chamber, and an aluminum sleeve with four septa. The modified rotor has a volume of 272 ml and has proved very suitable for rate-se
Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gr
An inexpensive apparatus is described for the preparation of large-volume convex exponential gradients for zonal rotors. The apparatus makes use of a constant volume mixing chamber, and efficient mixing of large volumes at high flow rates is obtained by the adaptation of an ordinary homogenizer. Exa