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A simple gel detection method of microbial tannin acyl hydrolase (EC 3.1.1.20)

✍ Scribed by C. Maity; P. K. Das Mohapatra; B. R. Pati; K. C. Mondal


Book ID
106564946
Publisher
Springer
Year
2008
Tongue
English
Weight
168 KB
Volume
25
Category
Article
ISSN
1573-0972

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✍ K.C. Mondal; D. Banerjee; M. Jana; B.R. Pati πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 49 KB

A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified