A simple enzymatic microdetermination of cytochrome b5 in erythrocytes

✍ Scribed by Masazumi Takeshita; Toshitsugu Yubisui; Kiyoo Tanishima; Yoshimasa Yoneyama

• Book ID: 102626098
• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 407 KB
• Volume: 107
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90387-5

✦ Synopsis

A simple enzymic method for the microscale determination of erythrocyte cytochrome b, is presented.

For assay of cytochrome b, the sodium nitrite-treated hemolysate was incubated in the presence of NADH and NADH-cytochrome b, reductase. and the reduction of methemoglobin was measured.

The rate of methemoglobin reduction was linearly dependent on the amount of cytochrome b,. When the red cells were stored at 4°C or methemoglobin-formed hemolysates were stored at 4°C or -20°C. there was no significant decline in the amount of cytochrome b, for periods of at least 2 weeks. Values for normal human subjects showed a mean of 0.70 IIZ 0.17 SE nmol/ml of red cells for males, 0.52 t 0.11 nmol/ml red cells for females, and 0.48 2 0.15 nmoliml red cells for umbilical cord bloods.